Basic Statistics
Measure | Value |
---|---|
Filename | NA12878_S1_L001_R2_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 388038711 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 624358 | 0.16090095712126 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCGTCG | 221350 | 0.0 | 49.95286 | 9 |
GAGCGTC | 254655 | 0.0 | 43.4568 | 8 |
AGAGCGT | 286930 | 0.0 | 38.694824 | 7 |
ATCGGAA | 299715 | 0.0 | 38.590275 | 1 |
TCGGAAG | 310870 | 0.0 | 37.061596 | 2 |
CGGAAGA | 323010 | 0.0 | 35.409363 | 3 |
AAGAGCG | 329355 | 0.0 | 34.62228 | 6 |
GAAGAGC | 581795 | 0.0 | 20.037188 | 5 |
CGCCGTA | 114035 | 0.0 | 17.917007 | 45-49 |
TCGCCGT | 126355 | 0.0 | 15.756337 | 40-44 |
GTCGCCG | 149945 | 0.0 | 15.523757 | 40-44 |
GCCGTAT | 133035 | 0.0 | 15.469274 | 45-49 |
CCGTATC | 142475 | 0.0 | 14.532856 | 45-49 |
TGGTCGC | 163635 | 0.0 | 14.162985 | 40-44 |
CGTATCA | 147990 | 0.0 | 14.051038 | 45-49 |
GGTCGCC | 168970 | 0.0 | 13.882279 | 40-44 |
GTGGTCG | 177070 | 0.0 | 13.278357 | 40-44 |
GGAAGAG | 902175 | 0.0 | 13.264691 | 4 |
TCTCGGT | 211740 | 0.0 | 11.285954 | 35-39 |
TCGGTGG | 216575 | 0.0 | 11.114338 | 35-39 |