FastQCFastQC Report
Mon 19 Jun 2017
NA12878_PCRFRee_20170509.MPS12342217-E10.4212.1.pair1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNA12878_PCRFRee_20170509.MPS12342217-E10.4212.1.pair1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490121034
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1404550.06.7021766145
ACACGTC7273500.06.248943145
TATGCCG1204550.06.232738145
CGTCTGA6433200.06.1763964145
TCTCGTA1517800.06.106607145
ACGTCTG7040900.05.905767145
CACGTCT7542750.05.8154716145
ATCTCGT1837900.05.7803755145
CGTATGC1546900.05.579477145
GCACACG8776050.05.517614145
CACACGT8620950.05.4706182145
GATCGGA11352300.05.4515243145
ATCGGAA11118850.05.4278116145
TCGGAAG10887050.05.416908145
CGGAAGA10701000.05.328242145
AGATCGG11831450.05.2711735145
ATGCCGT1425000.05.0651045145
GTCACTA5178700.05.0180326145
TCGTATG1738050.05.0033736145