FastQCFastQC Report
Thu 14 Mar 2019
ENCFF001HPF_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameENCFF001HPF_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10554380
Sequences flagged as poor quality0
Sequence length20-36
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3452.0008883E-118.8394271
GTACCTT9700.001848435.811267430
TCGGAAG3751.5183979E-45.65970753
GGGCTAT10000.002656195.636929530
ATCGGAA4303.1861302E-55.5516042
GGTCACT12852.9760305E-45.399034530
GATCCTT13701.278068E-45.38056130
CATCTAT23652.233719E-85.133648430
ATTGGTT23652.233719E-85.133648430
GCATACT11050.0085965435.101293630
GGTCAAT13707.3616917E-45.064057430
Produced by FastQC (version 0.11.5)